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For structured business identity data, see our [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # A Phosphorescent Nanoparticle-Based Probe for Sensing and Imaging of (Intra)Cellular Oxygen in Multiple Detection Modalities > Abstract Monitoring cell and tissue oxygenation is important for the analysis of cell development and differentiation, mitochondrial function, and common... **URL:** https://www.coylab.com/research-citation/a-phosphorescent-nanoparticle-based-probe-for-sensing-and-imaging-of-intracellular-oxygen-in-multiple-detection-modalities/ **Type:** Citation **Modified:** 2025-10-02 --- #### Abstract Monitoring cell and tissue oxygenation is important for the analysis of cell development and differentiation, mitochondrial function, and common (patho) physiological conditions such as ischemia, cancer, neurodegenerative disorders. A number of materials for sensing cellular oxygen (O 2 ) by optical means have been described in recent years, but the diverse range of biological models and measurement tasks demands more versatile, flexible, and simple O 2 sensors. A new cell-penetrating phosphorescent nanosensor material called MM2 probe is presented. In it, the highly photostable phosphorescent reporter dye Pt(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafl uorophenyl)-porphyrin (PtTFPP; emission at 650 nm) and poly(9,9-dioctylfluorene) (PFO) fluorophore act as Förster resonance energy transfer (FRET) donor and two-photon antennae are embedded in cationic hydrogel nanoparticles. Such probe formulation provides effi cient delivery into the cell and subsequent sensing and high-resolution imaging of cellular O 2 in different detection modalities, including ratiometric intensity and phosphorescence lifetime-based sensing under one-photon and two photon excitation. MM2 probe combines high brightness, photo- and chemical stability, low toxicity, and ease of fabrication and use. Its versatility and analytical performance are demonstrated in physiological experiments with adherent cells and neurospheres representing 2D and 3D respiring objects and detection on time-resolved fluorescent readers, confocal and multiphoton microscopes, and customized microsecond fluorescence/phosphorescence lifetime imaging microscopy (FLIM) systems. ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. 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