--- This page is part of [Coy Laboratory Products](https://www.coylab.com). For a complete overview of this site's content, entity data, and subject matter expertise, see our [AI Content Index](https://www.coylab.com/llms.txt) or [Full Content Archive](https://www.coylab.com/llms-full.txt). For structured business identity data, see our [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # Assessment of Cellular Oxygen Gradients with a Panel of Phosphorescent Oxygen-Sensitive Probes > Abstract The supply of oxygen (O2) to respiring tissue, cells, and mitochondria regulates metabolism, gene expression, and cell fate. Depending on the cell... **URL:** https://www.coylab.com/research-citation/assessment-of-cellular-oxygen-gradients-with-a-panel-of-phosphorescent-oxygen-sensitive-probes/ **Type:** Citation **Modified:** 2025-10-02 --- #### Abstract The supply of oxygen (O2) to respiring tissue, cells, and mitochondria regulates metabolism, gene expression, and cell fate. Depending on the cell type and mitochondrial function, O2 gradients between extra- and intracellular compartments may vary and play important physiological roles such as the regulation of activity of prolyl hydroxylases and adaptive responses to hypoxia. Here we present a new methodology for the analysis of localized O2 gradients in cultures of adherent cells, using three phosphorescent Pt-porphyrin based probes with different localization. One new O2 probe targeted to the cell membrane was developed and used together with existing MitoXpress and Nano2 probes to monitor mean pericellular (PC), extracellular (EC), and intracellular (IC) O2 concentrations, respectively. Mouse fibroblasts and neuronal PC12 cells cultured in standard microplates were stained with probes and measured on a commercial time-resolved fluorescence reader in phosphorescence lifetime mode. Respiring cells exposed to various levels of atmospheric O2 showed differences in oxygenation of their IC, PC, and EC compartments. Experiments with different cell numbers and modulation of respiration activity demonstrated that these gradients are dynamic and regulated by the O2 diffusion and consumption rate. The new method facilitates the assessment of such gradients. ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. [AI Content Index](https://www.coylab.com/llms.txt) | [Full Site Content](https://www.coylab.com/llms-full.txt) | [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card) ```json {"@context":"https://schema.org","@graph":[{"@type":"Organization","@id":"https://www.coylab.com/#organization","name":"Coy Laboratory Products","url":"https://www.coylab.com","description":"From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.","disambiguatingDescription":"Laboratory equipment manufacturing company specializing in vinyl glove boxes","logo":{"@type":"ImageObject","url":"https://coylab.com/wp-content/uploads/2025/06/coylab-header_logo-svg.svg"},"address":{"@type":"PostalAddress","streetAddress":"14500 Coy Drive","addressLocality":"Grass Lake","addressRegion":"MI","postalCode":"49240"},"telephone":"734.475.2200","email":"sales@coylab.com","foundingDate":"1969","areaServed":"Global","sameAs":["https://www.linkedin.com/company/107172311","https://maps.app.goo.gl/o2txKDMScV1pV7w59","https://www.pharmaceutical-tech.com/suppliers/coy-laboratory","https://craft.co/coy-laboratory-products-inc"],"founder":[{"@type":"Person","name":"Richard Coy"},{"@type":"Person","name":"Brian Coy","sameAs":"https://www.linkedin.com/in/brian-coy-5722515/"},{"@type":"Person","name":"Ashley Coy","sameAs":"https://www.linkedin.com/in/ashley-coy-6b22705/"}],"knowsAbout":[{"@type":"Thing","name":"laboratory equipment","sameAs":"https://www.wikidata.org/wiki/Q834028"},"vinyl anaerobic chambers","vinyl hypoxic chambers","laboratory glove boxes","laboratory chamber sensors",{"@type":"Thing","name":"life sciences","sameAs":"https://www.wikidata.org/wiki/Q864928"},{"@type":"Thing","name":"microbiology","sameAs":"https://www.wikidata.org/wiki/Q7193"},{"@type":"Thing","name":"medical research","sameAs":"https://www.wikidata.org/wiki/Q2752427"}]},{"@type":"WebSite","@id":"https://www.coylab.com/#website","name":"Coy Laboratory Products","url":"https://www.coylab.com","publisher":{"@id":"https://www.coylab.com/#organization"},"potentialAction":{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://www.coylab.com/?s={search_term_string}"},"query-input":"required name=search_term_string"},"hasPart":[{"@type":"DigitalDocument","name":"LLM Content Index","url":"https://www.coylab.com/llms.txt","encodingFormat":"text/markdown","description":"AI-readable index of site content, entity data, and subject matter expertise"},{"@type":"DigitalDocument","name":"LLM Full Content","url":"https://www.coylab.com/llms-full.txt","encodingFormat":"text/markdown","description":"Complete AI-readable site content"}]},{"@type":"ItemList","@id":"https://www.coylab.com/#site-navigation","name":"Site Navigation","itemListElement":[{"@type":"SiteNavigationElement","position":1,"name":"Products","url":"#"},{"@type":"SiteNavigationElement","position":2,"name":"Applications","url":"#"},{"@type":"SiteNavigationElement","position":3,"name":"Custom","url":"#"},{"@type":"SiteNavigationElement","position":4,"name":"Sales","url":"#"},{"@type":"SiteNavigationElement","position":5,"name":"Resources","url":"#"},{"@type":"SiteNavigationElement","position":6,"name":"Contact Us","url":"#"}]},{"@type":"BreadcrumbList","@id":"https://www.coylab.com/research-citation/assessment-of-cellular-oxygen-gradients-with-a-panel-of-phosphorescent-oxygen-sensitive-probes/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://www.coylab.com"},{"@type":"ListItem","position":2,"name":"Citations","item":"https://www.coylab.com/research-citations/"},{"@type":"ListItem","position":3,"name":"Hypoxic Chambers","item":"https://www.coylab.com/citation-category/hypoxic-chambers/"},{"@type":"ListItem","position":4,"name":"Assessment of Cellular Oxygen Gradients with a Panel of Phosphorescent Oxygen-Sensitive Probes"}]},{"@type":"WebPage","@id":"https://www.coylab.com/research-citation/assessment-of-cellular-oxygen-gradients-with-a-panel-of-phosphorescent-oxygen-sensitive-probes/#webpage","url":"https://www.coylab.com/research-citation/assessment-of-cellular-oxygen-gradients-with-a-panel-of-phosphorescent-oxygen-sensitive-probes/","name":"Assessment of Cellular Oxygen Gradients with a Panel of Phosphorescent Oxygen-Sensitive Probes | Coy Laboratory Products","isPartOf":{"@id":"https://www.coylab.com/#website"},"breadcrumb":{"@id":"https://www.coylab.com/research-citation/assessment-of-cellular-oxygen-gradients-with-a-panel-of-phosphorescent-oxygen-sensitive-probes/#breadcrumb"},"description":"Scientists around the world have relied on Coy chambers to conduct groundbreaking research into cancer growth, the human microbiome, c-diff., and more.","about":[{"@type":"Thing","name":"Hypoxic Chambers"}]},{"@type":"ScholarlyArticle","@id":"https://www.coylab.com/research-citation/assessment-of-cellular-oxygen-gradients-with-a-panel-of-phosphorescent-oxygen-sensitive-probes/#scholarlyarticle","headline":"Assessment of Cellular Oxygen Gradients with a Panel of Phosphorescent Oxygen-Sensitive Probes","url":"https://www.coylab.com/research-citation/assessment-of-cellular-oxygen-gradients-with-a-panel-of-phosphorescent-oxygen-sensitive-probes/","datePublished":"2025-09-18T15:59:48+00:00","dateModified":"2025-10-02T20:22:07+00:00","isPartOf":{"@id":"https://www.coylab.com/#website"},"description":"Scientists around the world have relied on Coy chambers to conduct groundbreaking research into cancer growth, the human microbiome, c-diff., and more.","author":{"@id":"https://www.coylab.com/author/buildcreate/#person"},"publisher":{"@id":"https://www.coylab.com/#organization"}},{"@type":"Person","@id":"https://www.coylab.com/author/buildcreate/#person","name":"buildcreate","url":"https://www.coylab.com/author/buildcreate/"}]} ```