--- This page is part of [Coy Laboratory Products](https://www.coylab.com). For a complete overview of this site's content, entity data, and subject matter expertise, see our [AI Content Index](https://www.coylab.com/llms.txt) or [Full Content Archive](https://www.coylab.com/llms-full.txt). For structured business identity data, see our [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates > Abstract A new online monitoring technique to measure the physiological parameters, dissolved oxygen (DO) and pH of microbial cultures in continuously... **URL:** https://www.coylab.com/research-citation/characterisation-of-operation-conditions-and-online-monitoring-of-physiological-culture-parameters-in-shaken-24-well-microtiter-plates/ **Type:** Citation **Modified:** 2025-09-26 --- #### Abstract A new online monitoring technique to measure the physiological parameters, dissolved oxygen (DO) and pH of microbial cultures in continuously shaken 24-well microtiter plates (MTP) is introduced. The new technology is based on immobilised fluorophores at the bottom of standard 24-well MTPs. The sensor MTP is installed in a sensor dish reader, which can be fixed on an orbital shaker. This approach allows real online measurements of physiological parameters during continuous shaking of cultures without interrupting mixing and mass transfer like currently available technologies do. The oxygen transfer conditions at one constant shaking frequency (250 1/min) and diameter (25 mm) was examined with the chemical sulphite oxidation method. Varied filling volumes (600–1,200 lL) of Escherichia coli cultures demonstrated the importance of sufficient oxygen transfer to the culture. Cultures with higher filling volumes were subjected to an oxygen limitation, which influenced the cell metabolism and prolongated the cultivation time. The effects could be clearly monitored by online DO and pH measurements. A further study of different media in an E. coli fermentation elucidated the different growth behaviour in response to the medium composition. The MTP fermentations correlated very well with parallel fermentations in shake flasks. The new technique gives valuable new insights into biological processes at a very small scale, thus enabling parallel experimentation and shorter development times in bioprocessing. ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. [AI Content Index](https://www.coylab.com/llms.txt) | [Full Site Content](https://www.coylab.com/llms-full.txt) | [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card) ```json {"@context":"https://schema.org","@graph":[{"@type":"Organization","@id":"https://www.coylab.com/#organization","name":"Coy Laboratory Products","url":"https://www.coylab.com","description":"From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.","disambiguatingDescription":"Laboratory equipment manufacturing company specializing in vinyl glove boxes","logo":{"@type":"ImageObject","url":"https://coylab.com/wp-content/uploads/2025/06/coylab-header_logo-svg.svg"},"address":{"@type":"PostalAddress","streetAddress":"14500 Coy Drive","addressLocality":"Grass Lake","addressRegion":"MI","postalCode":"49240"},"telephone":"734.475.2200","email":"sales@coylab.com","foundingDate":"1969","areaServed":"Global","sameAs":["https://www.linkedin.com/company/107172311","https://maps.app.goo.gl/o2txKDMScV1pV7w59","https://www.pharmaceutical-tech.com/suppliers/coy-laboratory","https://craft.co/coy-laboratory-products-inc"],"founder":[{"@type":"Person","name":"Richard Coy"},{"@type":"Person","name":"Brian Coy","sameAs":"https://www.linkedin.com/in/brian-coy-5722515/"},{"@type":"Person","name":"Ashley Coy","sameAs":"https://www.linkedin.com/in/ashley-coy-6b22705/"}],"knowsAbout":[{"@type":"Thing","name":"laboratory equipment","sameAs":"https://www.wikidata.org/wiki/Q834028"},"vinyl anaerobic chambers","vinyl hypoxic chambers","laboratory glove boxes","laboratory chamber sensors",{"@type":"Thing","name":"life sciences","sameAs":"https://www.wikidata.org/wiki/Q864928"},{"@type":"Thing","name":"microbiology","sameAs":"https://www.wikidata.org/wiki/Q7193"},{"@type":"Thing","name":"medical research","sameAs":"https://www.wikidata.org/wiki/Q2752427"}]},{"@type":"WebSite","@id":"https://www.coylab.com/#website","name":"Coy Laboratory Products","url":"https://www.coylab.com","publisher":{"@id":"https://www.coylab.com/#organization"},"potentialAction":{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://www.coylab.com/?s={search_term_string}"},"query-input":"required name=search_term_string"},"hasPart":[{"@type":"DigitalDocument","name":"LLM Content Index","url":"https://www.coylab.com/llms.txt","encodingFormat":"text/markdown","description":"AI-readable index of site content, entity data, and subject matter expertise"},{"@type":"DigitalDocument","name":"LLM Full Content","url":"https://www.coylab.com/llms-full.txt","encodingFormat":"text/markdown","description":"Complete AI-readable site content"}]},{"@type":"ItemList","@id":"https://www.coylab.com/#site-navigation","name":"Site Navigation","itemListElement":[{"@type":"SiteNavigationElement","position":1,"name":"Products","url":"#"},{"@type":"SiteNavigationElement","position":2,"name":"Applications","url":"#"},{"@type":"SiteNavigationElement","position":3,"name":"Custom","url":"#"},{"@type":"SiteNavigationElement","position":4,"name":"Sales","url":"#"},{"@type":"SiteNavigationElement","position":5,"name":"Resources","url":"#"},{"@type":"SiteNavigationElement","position":6,"name":"Contact Us","url":"#"}]},{"@type":"BreadcrumbList","@id":"https://www.coylab.com/research-citation/characterisation-of-operation-conditions-and-online-monitoring-of-physiological-culture-parameters-in-shaken-24-well-microtiter-plates/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://www.coylab.com"},{"@type":"ListItem","position":2,"name":"Citations","item":"https://www.coylab.com/research-citations/"},{"@type":"ListItem","position":3,"name":"Anaerobic Chambers","item":"https://www.coylab.com/citation-category/anaerobic-chambers/"},{"@type":"ListItem","position":4,"name":"Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates"}]},{"@type":"WebPage","@id":"https://www.coylab.com/research-citation/characterisation-of-operation-conditions-and-online-monitoring-of-physiological-culture-parameters-in-shaken-24-well-microtiter-plates/#webpage","url":"https://www.coylab.com/research-citation/characterisation-of-operation-conditions-and-online-monitoring-of-physiological-culture-parameters-in-shaken-24-well-microtiter-plates/","name":"Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates | Coy Laboratory Products","isPartOf":{"@id":"https://www.coylab.com/#website"},"breadcrumb":{"@id":"https://www.coylab.com/research-citation/characterisation-of-operation-conditions-and-online-monitoring-of-physiological-culture-parameters-in-shaken-24-well-microtiter-plates/#breadcrumb"},"description":"Scientists around the world have relied on Coy chambers to conduct groundbreaking research into cancer growth, the human microbiome, c-diff., and more.","about":[{"@type":"Thing","name":"Anaerobic Chambers"}]},{"@type":"ScholarlyArticle","@id":"https://www.coylab.com/research-citation/characterisation-of-operation-conditions-and-online-monitoring-of-physiological-culture-parameters-in-shaken-24-well-microtiter-plates/#scholarlyarticle","headline":"Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates","url":"https://www.coylab.com/research-citation/characterisation-of-operation-conditions-and-online-monitoring-of-physiological-culture-parameters-in-shaken-24-well-microtiter-plates/","datePublished":"2025-09-18T21:19:56+00:00","dateModified":"2025-09-26T16:16:43+00:00","isPartOf":{"@id":"https://www.coylab.com/#website"},"description":"Scientists around the world have relied on Coy chambers to conduct groundbreaking research into cancer growth, the human microbiome, c-diff., and more.","author":{"@id":"https://www.coylab.com/author/buildcreate/#person"},"publisher":{"@id":"https://www.coylab.com/#organization"}},{"@type":"Person","@id":"https://www.coylab.com/author/buildcreate/#person","name":"buildcreate","url":"https://www.coylab.com/author/buildcreate/"}]} ```