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For structured business identity data, see our [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # Hypoxia-inducible factor and nuclear factor kappa-B activation in blood–brain barrier endothelium under hypoxic/reoxygenation stress > Abstract This investigation focuses on transcription factor involvement in blood–brain barrier (BBB) endothelial cell-induced alterations under conditions... **URL:** https://www.coylab.com/research-citation/hypoxia-inducible-factor-and-nuclear-factor-kappa-b-activation-in-blood-brain-barrier-endothelium-under-hypoxic-reoxygenation-stress/ **Type:** Citation **Modified:** 2025-10-02 --- #### Abstract This investigation focuses on transcription factor involvement in blood–brain barrier (BBB) endothelial cell-induced alterations under conditions of hypoxia and post-hypoxia/reoxygenation (H/R), using established in vivo/ex vivo and in vitro BBB models. Protein/DNA array analyses revealed a correlation in key transcription factor activation during hypoxia and H/R, including NFjB and hypoxia-inducible factor (HIF)1. Electrophoretic mobility shift assays confirmed NFjB and HIF1 binding activity ex vivo and in vitro, under conditions of hypoxia and H/R. Hypoxia- and H/R-treated BBB endothelium showed increased HIF1a protein expression in both cytoplasmic and nuclear fractions, in ex vivo and in vitro models. Co-immunoprecipitation of HIF1a and HIF1b was shown in the nuclear fraction under conditions of hypoxia and H/R in both models. Hypoxia- and H/R-treated BBB endothelium showed increased expression of NFjB-p65 protein in both cytoplasmic and nuclear fractions. Co-immunoprecipitation of NFjB-p65. with NFjB-p50 was shown in the nuclear fraction under conditions of hypoxia and H/R in the ex vivo model, and after H/R in the in vitro model. These data offer novel avenues in which to alter and/or investigate BBB activity across model systems and to further our understanding of upstream regulators during hypoxia and H/R. ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. 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