--- This page is part of [Coy Laboratory Products](https://www.coylab.com). For a complete overview of this site's content, entity data, and subject matter expertise, see our [AI Content Index](https://www.coylab.com/llms.txt) or [Full Content Archive](https://www.coylab.com/llms-full.txt). For structured business identity data, see our [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # Kinetic Analysis of the Oxidative Conversion of the [4Fe-4S]2 Cluster of FNR to a [2Fe-2S]2+ Cluster > Abstract The ability of FNR to sense and respond to cellular O2 levels depends on its [4Fe-4S]2 cluster. In the presence of O2, the [4Fe-4S]2 cluster is... **URL:** https://www.coylab.com/research-citation/kinetic-analysis-of-the-oxidative-conversion-of-the-4fe-4s2-cluster-of-fnr-to-a-2fe-2s2-cluster/ **Type:** Citation **Modified:** 2025-09-26 --- #### Abstract The ability of FNR to sense and respond to cellular O2 levels depends on its [4Fe-4S]2 cluster. In the presence of O2, the [4Fe-4S]2 cluster is converted to a [2Fe-2S]2 cluster, which inactivates FNR as a transcriptional regulator. In this study, we demonstrate that 2 Fe2 ions are released from the reaction of O2 with the [4Fe-4S]2 cluster. Fe2 release was then used as an assay of reaction progress to investigate the rate of [4Fe-4S]2 to [2Fe-2S]2 cluster conversion in vitro. We also found that there was no detectable difference in the rate of O2-induced cluster conversion for FNR free in solution compared to its DNA bound form. In addition, the rate of FNR inactivation was monitored in vivo by measuring the rate at which transcriptional regulation by FNR is lost upon the exposure of cells to O2; a comparison of the in vitro and in vivo rates of conversion suggests that O2-induced cluster conversion is sufficient to explain FNR inactivation in cells. FNR protein levels were also compared for cells grown under aerobic and anaerobic conditions. ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. 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