--- This page is part of [Coy Laboratory Products](https://www.coylab.com). For a complete overview of this site's content, entity data, and subject matter expertise, see our [AI Content Index](https://www.coylab.com/llms.txt) or [Full Content Archive](https://www.coylab.com/llms-full.txt). For structured business identity data, see our [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # Supporting Online Material for An E3 Ligase Possessing an Iron-Responsive Hemerythrin Domain Is a Regulator of Iron Homeostasis > Abstract Non-tumorigenic immortalized human bronchial epithelial HBEC-30 cells ((S1); kindly provided by M. White), were grown in keratinocyte serum free... **URL:** https://www.coylab.com/research-citation/supporting-online-material-for-an-e3-ligase-possessing-an-iron-responsive-hemerythrin-domain-is-a-regulator-of-iron-homeostasis/ **Type:** Citation **Modified:** 2025-10-02 --- #### Abstract Non-tumorigenic immortalized human bronchial epithelial HBEC-30 cells ((S1); kindly provided by M. White), were grown in keratinocyte serum free medium supplemented with recombinant epidermal growth factor and bovine pituitary extract (Gibco). All other cell lines were grown in Dulbecco’s Modified High Glucose Eagle Medium (HyClone). Tetracycline inducible cells were supplemented with 10% tetracycline-free fetal bovine serum (Clontech) while all other cells were supplemented with 10% fetal bovine serum (Atlanta Biologicals). Low (1%) O2 incubations were carried out in a hypoxic chamber (Coy Laboratory Products). Transient transfections of plasmid DNA were carried out using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Typically, 5×104 cells were plated per well of a 24-well plate and incubated for 16 hr followed by transfection with 500 ng of DNA. Neomycin-resistant cells stably expressing epitope-tagged IRP2 or FBXL5 proteins were isolated over two rounds of clonal selection in the presence of 400 μg/mL G418 (Research Products Inc). IRP1 and IRP13C>3S Flp-In T-Rex HEK 293 cells were kindly provided by R. Eisenstein. ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. 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