--- This page is part of [Coy Laboratory Products](https://www.coylab.com). For a complete overview of this site's content, entity data, and subject matter expertise, see our [AI Content Index](https://www.coylab.com/llms.txt) or [Full Content Archive](https://www.coylab.com/llms-full.txt). For structured business identity data, see our [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card). --- # The CcpA Protein Is Necessary for Efficient Sporulation and Enterotoxin Gene (cpe) Regulation in Clostridium perfringens > Abstract Clostridium perfringens is the cause of several human diseases, including gas gangrene (clostridial myonecrosis), enteritis necroticans,... **URL:** https://www.coylab.com/research-citation/the-ccpa-protein-is-necessary-for-efficient-sporulation-and-enterotoxin-gene-cpe-regulation-in-clostridium-perfringens/ **Type:** Citation **Modified:** 2025-09-26 --- #### Abstract Clostridium perfringens is the cause of several human diseases, including gas gangrene (clostridial myonecrosis), enteritis necroticans, antibiotic-associated diarrhea, and acute food poisoning. The symptoms of antibiotic-associated diarrhea and acute food poisoning are due to sporulation-dependent production of C. perfringens enterotoxin encoded by the cpe gene. Glucose is a catabolite repressor of sporulation by C. perfringens. In order to identify the mechanism of catabolite repression by glucose, a mutation was introduced into the ccpAgene of C. perfringens by conjugational transfer of a nonreplicating plasmid into C. perfringens, which led to inactivation of the ccpA gene by homologous recombination. CcpA is a transcriptional regulator known to mediate catabolite repression in a number of low-GC-content gram-positive bacteria, of which C. perfringens is a member. The ccpA mutant strain sporulated at a 60-fold lower efficiency than the wild-type strain in the absence of glucose. In the presence of 5 mM glucose, sporulation was repressed about 2,000-fold in t hewild-type strain and 800-fold in the ccpA mutant strain compared to sporulation levels for the same strains grown in the absence of glucose. Therefore, while CcpA is necessary for efficient sporulation in C. perfringens, glucose-mediated catabolite repression of sporulation is not due to the activity of CcpA. Transcription of the cpe gene was measured in the wild-type and ccpA mutant strains grown in sporulation medium by using a cpe-gusA fusion (gusA is an Escherichia coli gene encoding the enzyme -glucuronidase). In the exponential growth phase, cpe transcription was two times higher in the ccpA mutant strain than in the wild-type strain. Transcription of cpe was highly induced during the entry into stationary phase in wild-type cells but was not induced in the ccpA mutant strain. Glucose repressed cpe transcription in both the wild-type and ccpA mutant strain. Therefore, CcpA appears to act as a repressor of cpe transcription in exponential growth but is required for efficient sporulation and cpe transcription upon entry into stationary phase. CcpA was also required for maximum synthesis of collagenase (kappa toxin) and acted as a repressor of polysaccharide capsule synthesis in the presence of glucose, but it did not regulate synthesis of the phospholipase PLC (alpha toxin). ## Site Description From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs. --- **About this site:** Coy Laboratory Products — From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.. [AI Content Index](https://www.coylab.com/llms.txt) | [Full Site Content](https://www.coylab.com/llms-full.txt) | [Entity Card](https://www.coylab.com/wp-json/bc-geodesic/v1/entity-card) ```json {"@context":"https://schema.org","@graph":[{"@type":"Organization","@id":"https://www.coylab.com/#organization","name":"Coy Laboratory Products","url":"https://www.coylab.com","description":"From academia to industry, Coy Labs chambers have powered discovery in labs around the world for over five decades—from custom chambers built to spec, to trusted workhorses in high-stakes microbiology labs.","disambiguatingDescription":"Laboratory equipment manufacturing company specializing in vinyl glove boxes","logo":{"@type":"ImageObject","url":"https://coylab.com/wp-content/uploads/2025/06/coylab-header_logo-svg.svg"},"address":{"@type":"PostalAddress","streetAddress":"14500 Coy Drive","addressLocality":"Grass Lake","addressRegion":"MI","postalCode":"49240"},"telephone":"734.475.2200","email":"sales@coylab.com","foundingDate":"1969","areaServed":"Global","sameAs":["https://www.linkedin.com/company/107172311","https://maps.app.goo.gl/o2txKDMScV1pV7w59","https://www.pharmaceutical-tech.com/suppliers/coy-laboratory","https://craft.co/coy-laboratory-products-inc"],"founder":[{"@type":"Person","name":"Richard Coy"},{"@type":"Person","name":"Brian Coy","sameAs":"https://www.linkedin.com/in/brian-coy-5722515/"},{"@type":"Person","name":"Ashley Coy","sameAs":"https://www.linkedin.com/in/ashley-coy-6b22705/"}],"knowsAbout":[{"@type":"Thing","name":"laboratory equipment","sameAs":"https://www.wikidata.org/wiki/Q834028"},"vinyl anaerobic chambers","vinyl hypoxic chambers","laboratory glove boxes","laboratory chamber sensors",{"@type":"Thing","name":"life sciences","sameAs":"https://www.wikidata.org/wiki/Q864928"},{"@type":"Thing","name":"microbiology","sameAs":"https://www.wikidata.org/wiki/Q7193"},{"@type":"Thing","name":"medical research","sameAs":"https://www.wikidata.org/wiki/Q2752427"}]},{"@type":"WebSite","@id":"https://www.coylab.com/#website","name":"Coy Laboratory Products","url":"https://www.coylab.com","publisher":{"@id":"https://www.coylab.com/#organization"},"potentialAction":{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://www.coylab.com/?s={search_term_string}"},"query-input":"required name=search_term_string"},"hasPart":[{"@type":"DigitalDocument","name":"LLM Content Index","url":"https://www.coylab.com/llms.txt","encodingFormat":"text/markdown","description":"AI-readable index of site content, entity data, and subject matter expertise"},{"@type":"DigitalDocument","name":"LLM Full Content","url":"https://www.coylab.com/llms-full.txt","encodingFormat":"text/markdown","description":"Complete AI-readable site content"}]},{"@type":"ItemList","@id":"https://www.coylab.com/#site-navigation","name":"Site Navigation","itemListElement":[{"@type":"SiteNavigationElement","position":1,"name":"Products","url":"#"},{"@type":"SiteNavigationElement","position":2,"name":"Applications","url":"#"},{"@type":"SiteNavigationElement","position":3,"name":"Custom","url":"#"},{"@type":"SiteNavigationElement","position":4,"name":"Sales","url":"#"},{"@type":"SiteNavigationElement","position":5,"name":"Resources","url":"#"},{"@type":"SiteNavigationElement","position":6,"name":"Contact Us","url":"#"}]},{"@type":"BreadcrumbList","@id":"https://www.coylab.com/research-citation/the-ccpa-protein-is-necessary-for-efficient-sporulation-and-enterotoxin-gene-cpe-regulation-in-clostridium-perfringens/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://www.coylab.com"},{"@type":"ListItem","position":2,"name":"Citations","item":"https://www.coylab.com/research-citations/"},{"@type":"ListItem","position":3,"name":"Anaerobic Chambers","item":"https://www.coylab.com/citation-category/anaerobic-chambers/"},{"@type":"ListItem","position":4,"name":"The CcpA Protein Is Necessary for Efficient Sporulation and Enterotoxin Gene (cpe) Regulation in Clostridium perfringens"}]},{"@type":"WebPage","@id":"https://www.coylab.com/research-citation/the-ccpa-protein-is-necessary-for-efficient-sporulation-and-enterotoxin-gene-cpe-regulation-in-clostridium-perfringens/#webpage","url":"https://www.coylab.com/research-citation/the-ccpa-protein-is-necessary-for-efficient-sporulation-and-enterotoxin-gene-cpe-regulation-in-clostridium-perfringens/","name":"The CcpA Protein Is Necessary for Efficient Sporulation and Enterotoxin Gene (cpe) Regulation in Clostridium perfringens | Coy Laboratory Products","isPartOf":{"@id":"https://www.coylab.com/#website"},"breadcrumb":{"@id":"https://www.coylab.com/research-citation/the-ccpa-protein-is-necessary-for-efficient-sporulation-and-enterotoxin-gene-cpe-regulation-in-clostridium-perfringens/#breadcrumb"},"description":"Scientists around the world have relied on Coy chambers to conduct groundbreaking research into cancer growth, the human microbiome, c-diff., and more.","about":[{"@type":"Thing","name":"Anaerobic Chambers"}]},{"@type":"ScholarlyArticle","@id":"https://www.coylab.com/research-citation/the-ccpa-protein-is-necessary-for-efficient-sporulation-and-enterotoxin-gene-cpe-regulation-in-clostridium-perfringens/#scholarlyarticle","headline":"The CcpA Protein Is Necessary for Efficient Sporulation and Enterotoxin Gene (cpe) Regulation in Clostridium perfringens","url":"https://www.coylab.com/research-citation/the-ccpa-protein-is-necessary-for-efficient-sporulation-and-enterotoxin-gene-cpe-regulation-in-clostridium-perfringens/","datePublished":"2025-09-15T16:50:08+00:00","dateModified":"2025-09-26T16:16:45+00:00","isPartOf":{"@id":"https://www.coylab.com/#website"},"description":"Scientists around the world have relied on Coy chambers to conduct groundbreaking research into cancer growth, the human microbiome, c-diff., and more.","author":{"@id":"https://www.coylab.com/author/buildcreate/#person"},"publisher":{"@id":"https://www.coylab.com/#organization"}},{"@type":"Person","@id":"https://www.coylab.com/author/buildcreate/#person","name":"buildcreate","url":"https://www.coylab.com/author/buildcreate/"}]} ```